investigation to see how enzyme activity is affected by temperature
In this investigation I will mix the enzyme protease with milk and see how long it takes for the solution to go clear. Im going to do the experiment several times at different temperatures to see if it effects the enzyme activity. ... The only thing that will change is the temperature of each experiment. ... If the stomach conditions are too acidic the active site becomes damaged, so the enzyme doesnt work as well. If the temperature is too high then the active site becomes denatured, this stops the enzyme working too. If the temperature is too low, the enzymes are not as active, so the chances of an enzyme locking onto a food molecule is slim. ... I will make sure they are the same temperature, then I will mix them together. ... I will do three tests at each temperature, and work out an average time from them to make it a fair experiment. My temperature range is 20, 30, 40, 50, and 70°C. ... I think this because average body temperature is between these times and the enzymes are designed to work inside the body at body temperature.