Papers > Science > effect of a substrate concentration on catalase
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effect of a substrate concentration on catalase
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done with a strong acid and a strong base of equal concentrations using microscale techniques. The chemicals used in this experiment were chosen because they react in a one-to-one stoichiometric mole ratio. Since you know precisely the concentration of base used, you can calculate the number of moles of base used. Because of the mole ratio, this is also the number of moles of acid used. Furthermore, from the volume of acid used, you can calculate the concentration or strength of the acid. Since the same pipet was used for both acid and base, calibration of this pipet is unnecessary. Macid = ( Mbase x # drops base)/# drops acid Go to Top Procedure Select a Beral pipet to use for the reagents. Fill the pipet with distilled water and then squeeze dry. Fill the pipet with a small portion of acid. Expel in the sink. Fill again with acid and expel in the sink. Fill the pipet with acid. Select a 24-well plate for the experiment. Carefully transfer 50 drops of acid to each of 3 wells. Add 1 or 2 drops of phenolphthalein indicator to each of the wells. Squeeze the pipet dry. Rinse the pipet 3 times with small portions of distilled water. Discard the rinse at the sink. Fill the pipet with a small portion of base. Rinse. Expel. Repeat the rinsing procedure. Fill the pipet. (Instead, you may wish to use the two-piece device consisting of a plastic bulb and a pipet tip described above.) 1 Over 600 people bought a mobile phone this week. 2 I am going on holiday in 10 days.
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Paper Information
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Title: effect of a substrate concentration on catalase
Words: 1326
Rating: None
Pages: 5.3
submitted by: Danniella
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