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Papers > Science > Effect Of Substrate Concentration On The Activity Of Catalase
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Effect Of Substrate Concentration On The Activity Of Catalase |
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Effect Of Substrate Concentration On The Activity Of Catalase AIM This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase. PREDICTION I predict that as the substrate concentration increases, the rate of reaction will go up at a directly proportional rate until the solution becomes saturated with the substrate hydrogen peroxide. When this saturation point is reached, then adding extra substrate will make no difference. The rate steadily increases when more substrate is added because more of the active sites of the enzyme are being used which results in more reactions so the required amount of oxygen is made more quickly. Once the amount of substrate molecules added exceeds the number of active sites available then the rate of reaction will no longer go up. This is because the maximum number of reactions are being done at once so any extra substrate molecules have to wait until some of the active sites become available. BACKGROUND INFORMATION Enzymes such as Catalase are protein molecules which are found in living cells. ... Catalase is an enzyme found in food such as potato and liver. ... Catalase speeds up the decomposition of Hydrogen Peroxide into water and oxygen as shown in the equations below. ... Yeast Catalase 4. ... Tap Water METHOD To test out how the concentration of hydrogen peroxide affects the rate of reaction first set up the apparatus below. ... Add 4cm3 of hydrogen peroxide solution at a concentration of 20% to the other test tube. ... The 0% concentration of hydrogen peroxide solution is done as a control solution to show that at 0% concentration no reaction occurs. ... Concentration Of Hydrogen Peroxide Volume Of Hydrogen Peroxide (cm3) Volume Of Water (cm3) 20% 4 0 16% 3. ... Hydrogen Peroxide Concentration 0% 4% 8% 10% 12% 16% 20% Time Taken (Test 1) Time Taken (Test 2) Time Taken (Test 3) Average of the Tests Rate The rate can then be worked out by Rate=30/Average Time This gives the rate in cm3 of oxygen produced per second, this is because I am timing how long it takes to produce 30cm3 of oxygen. |
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Title: Effect Of Substrate Concentration On The Activity Of Catalase
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