Papers > Technology > Cell Fractionation ªExtraction of mitochondria and illustration of electron transport activity in this cellular organelles
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Cell Fractionation ªExtraction of mitochondria and illustration of electron transport activity in this cellular organelles
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... The objective of cell fractionation by means of tissue homogenize is to cause cell (pig liver tissue) disruption and extract cell constituent¡ªmitochondria. ... The objective of centrifugation is to separate different cell constituents by different speed of centrifugation, so that to collect and assay the electron transport property of mitochondria. ... The clear evidence of electron transport activity is the changes in color in tubes containing -phenylenediamine. ... The mitochondrial electron carrier cytochrome c is reduced through the addition of the electrons from -phenylenediamine. ... The pigment produced serves as the indicator of electron transport. ... Azide inhibited the electron transport by comparing tube C with tube B. ... By comparing tube C with tube B, it is clear to see that azide blocks transport of electron from cytochrome a to oxygen, which cause the differences in degree of color.
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Title: Cell Fractionation ªExtraction of mitochondria and illustration of electron transport activity in this cellular organelles
Words: 661
Rating: None
Pages: 2.6
submitted by: lovelyxizi
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